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Combine gently just before transferring cells to some conical tube containing 10 mL of pre-warmed clean medium. Notice: Clean the pipette tip and cryovial Along with the wash medium at the very least 3 times that will help improve Restoration.
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It's important to quench the dissociation reagent working with FACS buffer or prevent medium, by including no less than the same or double the amount with the dissociation reagent.
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Heading again into the plate, rinse Every properly with one mL of FACS 줄기세포 지방이식 buffer and transfer the amount into the fifteen mL tube. Take note: Keep cell suspension on ice immediately 지방이식 after transfer towards the tube until finally able to run FACS.
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Notes: Use pipette ideas to Carefully scratch The underside of each effectively so as to lift the monolayer off, then dissociate the cell clumps into just one-cell suspension by pipetting up and down five - ten times.
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